CRISPR/Cas9-Mediated Disruption of Reveals Expansin as a Key Susceptibility Factor for Citrus Canker Disease

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dc.citation.journaltitleMOLECULAR PLANT-MICROBE INTERACTIONS
dc.creatorSOUZA-NETO, R. R.
dc.creatorCAVALCANTE, L. N.
dc.creatorCARVALHO, I. G. B.
dc.creatorCURTOLO, M.
dc.creatorBENEDETTI, C. E.
dc.creatorTAKITA, M. A.
dc.creatorWANG, N.
dc.creatorSOUZA, A. A.
dc.date.accessioned2026-05-05T17:56:50Z
dc.date.issued2025
dc.description.resumoThe Citrus sinensis LATERAL ORGAN BOUNDERIES 1 (CsLOB1) gene, which is directly induced by the Xanthomonas citri subsp. citri effector PthA4, functions as a transcription factor and citrus canker susceptibility (S) gene. Genome editing of CsLOB1 has been shown to confer resistance to citrus canker disease. Previous studies revealed that the citrus CsLOB1-INDUCED EXPANSIN 1 gene (CsLIEXP1) is highly and directly upregulated by CsLOB1 in Xanthomonas citri subsp. citri-infected plants. Because expansins are associated with cell wall loosening, potentially facilitating bacterial colonization, the CsLOB1-dependent activation of CsLIEXP1 is thought to contribute to canker symptoms and disease progression. Thus, CsLIEXP1 likely represents a critical canker susceptibility gene. In this study, we employed CRISPR/Cas9 to disrupt the function of CsLIEXP1 by modifying its corresponding coding region in Citrus sinensis cultivar ‘Hamlin’ and evaluated the postinfection responses of edited plants. DNA sequencing confirmed the edition of the CsLIEXP1-edited plant, which exhibited 26.47% of CsLIEXP1 edited sequences. Furthermore, CsLIEXP1 protein accumulation was reduced in CsLIEXP1-edited plants compared with the wild type when infected with X. citri. Leaves of edited plants inoculated with X. citri showed significantly fewer canker symptoms, with lesions limited to the site of bacterial inoculation and less pronounced cellular hypertrophy compared with control plants. Our results show that CsLIEXP1 is a citrus canker S gene that acts downstream of CsLOB1, thus providing new insights into plant-pathogen interactions.
dc.format.extent1-44
dc.format.issue4
dc.identifier.citationDE SOUZA-NETO, REINALDO RODRIGUES ; CAVALCANTE, LIDIA NASCIMENTO ; CARVALHO, ISIS GABRIELA BARBOSA ; CURTOLO, MAIARA ; BENEDETTI, CELSO EDUARDO ; TAKITA, MARCO AURELIO ; WANG, NIAN ; DE SOUZA, ALESSANDRA ALVES . CRISPR/Cas9-Mediated Disruption of Reveals Expansin as a Key Susceptibility Factor for Citrus Canker Disease. MOLECULAR PLANT-MICROBE INTERACTIONS, v. 4, p. 1-44, 2025. https://doi.org/10.1094/MPMI-12-24-0151-R
dc.identifier.doihttp://dx.doi.org/10.1094/MPMI-12-24-0151-R
dc.identifier.urihttps://repositorio.iac.sp.gov.br/handle/iac/47
dc.subjectCRISPR/Cas, genome editing, sweet orange, Xanthomonas citri
dc.titleCRISPR/Cas9-Mediated Disruption of Reveals Expansin as a Key Susceptibility Factor for Citrus Canker Disease

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